opal 480 Search Results


akoya spectra dapi  (Akoya Biosciences)
96
Akoya Biosciences akoya spectra dapi
Transient upregulation of SA‐β‐gal activity, p16, and 21‐positive cells during wound healing in young but not in old mice. (A) Representative immunofluorescence images of wound tissue sections from young mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with <t>DAPI</t> (blue) to visualize nuclei. Epi = epidermis, der = dermis for reference. (B) Representative immunofluorescence images of wound tissue sections from old mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with DAPI (blue). (C) Quantification of the percentage of SA‐β‐gal‐positive cells in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (D) Quantification of p16 mRNA relative expression in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (E, F) Representative immunofluorescence images demonstrating p21 (red) and DAPI (blue) staining in wound tissue from young (E) and aged (F) mice at 0, 6, 12, 18, and 24 days postwounding. The epidermis (epi) and dermis (der) are indicated for reference. (G) Quantification of the percentage of p21‐positive cells in wound tissue from young and old mice over the wound healing time course. (H) qRT‐PCR analysis of p21 gene expression in wound tissue from young and aged mice over the wound healing time course, shown as relative expression to β‐Actin. N = 5 per age group per time point. * p < 0.05, ** p < 0.01.
Akoya Spectra Dapi, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
akoya spectra dapi - by Bioz Stars, 2026-05
96/100 stars
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opal 480  (Quanterix)
98
Quanterix opal 480
Transient upregulation of SA‐β‐gal activity, p16, and 21‐positive cells during wound healing in young but not in old mice. (A) Representative immunofluorescence images of wound tissue sections from young mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with <t>DAPI</t> (blue) to visualize nuclei. Epi = epidermis, der = dermis for reference. (B) Representative immunofluorescence images of wound tissue sections from old mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with DAPI (blue). (C) Quantification of the percentage of SA‐β‐gal‐positive cells in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (D) Quantification of p16 mRNA relative expression in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (E, F) Representative immunofluorescence images demonstrating p21 (red) and DAPI (blue) staining in wound tissue from young (E) and aged (F) mice at 0, 6, 12, 18, and 24 days postwounding. The epidermis (epi) and dermis (der) are indicated for reference. (G) Quantification of the percentage of p21‐positive cells in wound tissue from young and old mice over the wound healing time course. (H) qRT‐PCR analysis of p21 gene expression in wound tissue from young and aged mice over the wound healing time course, shown as relative expression to β‐Actin. N = 5 per age group per time point. * p < 0.05, ** p < 0.01.
Opal 480, supplied by Quanterix, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/opal 480/product/Quanterix
Average 98 stars, based on 1 article reviews
opal 480 - by Bioz Stars, 2026-05
98/100 stars
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opal 480  (Innate Pharma)
90
Innate Pharma opal 480
Transient upregulation of SA‐β‐gal activity, p16, and 21‐positive cells during wound healing in young but not in old mice. (A) Representative immunofluorescence images of wound tissue sections from young mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with <t>DAPI</t> (blue) to visualize nuclei. Epi = epidermis, der = dermis for reference. (B) Representative immunofluorescence images of wound tissue sections from old mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with DAPI (blue). (C) Quantification of the percentage of SA‐β‐gal‐positive cells in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (D) Quantification of p16 mRNA relative expression in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (E, F) Representative immunofluorescence images demonstrating p21 (red) and DAPI (blue) staining in wound tissue from young (E) and aged (F) mice at 0, 6, 12, 18, and 24 days postwounding. The epidermis (epi) and dermis (der) are indicated for reference. (G) Quantification of the percentage of p21‐positive cells in wound tissue from young and old mice over the wound healing time course. (H) qRT‐PCR analysis of p21 gene expression in wound tissue from young and aged mice over the wound healing time course, shown as relative expression to β‐Actin. N = 5 per age group per time point. * p < 0.05, ** p < 0.01.
Opal 480, supplied by Innate Pharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/opal 480/product/Innate Pharma
Average 90 stars, based on 1 article reviews
opal 480 - by Bioz Stars, 2026-05
90/100 stars
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opal 480  (Vectra Laboratories)
90
Vectra Laboratories opal 480
Transient upregulation of SA‐β‐gal activity, p16, and 21‐positive cells during wound healing in young but not in old mice. (A) Representative immunofluorescence images of wound tissue sections from young mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with <t>DAPI</t> (blue) to visualize nuclei. Epi = epidermis, der = dermis for reference. (B) Representative immunofluorescence images of wound tissue sections from old mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with DAPI (blue). (C) Quantification of the percentage of SA‐β‐gal‐positive cells in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (D) Quantification of p16 mRNA relative expression in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (E, F) Representative immunofluorescence images demonstrating p21 (red) and DAPI (blue) staining in wound tissue from young (E) and aged (F) mice at 0, 6, 12, 18, and 24 days postwounding. The epidermis (epi) and dermis (der) are indicated for reference. (G) Quantification of the percentage of p21‐positive cells in wound tissue from young and old mice over the wound healing time course. (H) qRT‐PCR analysis of p21 gene expression in wound tissue from young and aged mice over the wound healing time course, shown as relative expression to β‐Actin. N = 5 per age group per time point. * p < 0.05, ** p < 0.01.
Opal 480, supplied by Vectra Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/opal 480/product/Vectra Laboratories
Average 90 stars, based on 1 article reviews
opal 480 - by Bioz Stars, 2026-05
90/100 stars
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opal polaris 480  (GeneWorks)
90
GeneWorks opal polaris 480
Transient upregulation of SA‐β‐gal activity, p16, and 21‐positive cells during wound healing in young but not in old mice. (A) Representative immunofluorescence images of wound tissue sections from young mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with <t>DAPI</t> (blue) to visualize nuclei. Epi = epidermis, der = dermis for reference. (B) Representative immunofluorescence images of wound tissue sections from old mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with DAPI (blue). (C) Quantification of the percentage of SA‐β‐gal‐positive cells in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (D) Quantification of p16 mRNA relative expression in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (E, F) Representative immunofluorescence images demonstrating p21 (red) and DAPI (blue) staining in wound tissue from young (E) and aged (F) mice at 0, 6, 12, 18, and 24 days postwounding. The epidermis (epi) and dermis (der) are indicated for reference. (G) Quantification of the percentage of p21‐positive cells in wound tissue from young and old mice over the wound healing time course. (H) qRT‐PCR analysis of p21 gene expression in wound tissue from young and aged mice over the wound healing time course, shown as relative expression to β‐Actin. N = 5 per age group per time point. * p < 0.05, ** p < 0.01.
Opal Polaris 480, supplied by GeneWorks, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/opal polaris 480/product/GeneWorks
Average 90 stars, based on 1 article reviews
opal polaris 480 - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


Transient upregulation of SA‐β‐gal activity, p16, and 21‐positive cells during wound healing in young but not in old mice. (A) Representative immunofluorescence images of wound tissue sections from young mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with DAPI (blue) to visualize nuclei. Epi = epidermis, der = dermis for reference. (B) Representative immunofluorescence images of wound tissue sections from old mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with DAPI (blue). (C) Quantification of the percentage of SA‐β‐gal‐positive cells in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (D) Quantification of p16 mRNA relative expression in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (E, F) Representative immunofluorescence images demonstrating p21 (red) and DAPI (blue) staining in wound tissue from young (E) and aged (F) mice at 0, 6, 12, 18, and 24 days postwounding. The epidermis (epi) and dermis (der) are indicated for reference. (G) Quantification of the percentage of p21‐positive cells in wound tissue from young and old mice over the wound healing time course. (H) qRT‐PCR analysis of p21 gene expression in wound tissue from young and aged mice over the wound healing time course, shown as relative expression to β‐Actin. N = 5 per age group per time point. * p < 0.05, ** p < 0.01.

Journal: Aging Cell

Article Title: Diminished and Altered Cellular Senescence Response in Delayed Wound Healing of Aging

doi: 10.1111/acel.70493

Figure Lengend Snippet: Transient upregulation of SA‐β‐gal activity, p16, and 21‐positive cells during wound healing in young but not in old mice. (A) Representative immunofluorescence images of wound tissue sections from young mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with DAPI (blue) to visualize nuclei. Epi = epidermis, der = dermis for reference. (B) Representative immunofluorescence images of wound tissue sections from old mice at days 0, 6, 12, 18, and 24 postwounding, stained for SA‐β‐gal activity (green) and counterstained with DAPI (blue). (C) Quantification of the percentage of SA‐β‐gal‐positive cells in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (D) Quantification of p16 mRNA relative expression in the wound tissue of young and old mice at days 0, 6, 12, 18, and 24 postwounding. (E, F) Representative immunofluorescence images demonstrating p21 (red) and DAPI (blue) staining in wound tissue from young (E) and aged (F) mice at 0, 6, 12, 18, and 24 days postwounding. The epidermis (epi) and dermis (der) are indicated for reference. (G) Quantification of the percentage of p21‐positive cells in wound tissue from young and old mice over the wound healing time course. (H) qRT‐PCR analysis of p21 gene expression in wound tissue from young and aged mice over the wound healing time course, shown as relative expression to β‐Actin. N = 5 per age group per time point. * p < 0.05, ** p < 0.01.

Article Snippet: Nuclei were counterstained with Akoya Spectra DAPI (FP1490, Akoya Biosciences), and slides were mounted with ProLong Gold Antifade Mountant ( P36930 , Invitrogen, Waltham, MA, USA).

Techniques: Activity Assay, Immunofluorescence, Staining, Expressing, Quantitative RT-PCR, Gene Expression